Figure 5. Human umbilical vein endothelial cells (HUVECs) were transfected with SIRT1 siRNA and miR-383 suppressor for 24 h prior to 48 h activation by treatment with high glucose (HG). A and B, Representative immunoblot and quantitative evaluation of SIRT1 in HUVECs. C, Reactive oxygen species (ROS) inside the cells was assessed by oxidation of H2DCFDA to DCF. D, Activity of superoxide dismutase (SOD1) and E, catalase (CAT) in groups. F, Flow cytometry analysis of cell death. Data are reported as means±SE of 3 independent experiments. **P<0.01 vs control; #P<0.05 vs negative control (NC)+HG group; $P<0.05 vs anti-miR-383 group (ANOVA and Tukey's post hoc test).
