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. 2019 Jun 14;16(2):334–346. doi: 10.1080/15548627.2019.1628539

Figure 4.

Figure 4.

GAS Nga inhibits PIK3C3 complex-dependent autophagosome formation and promotes intracellular proliferation. (a and b) HeLa cells stably expressing GFP-LC3 were infected with GAS JRS4 wild-type or indicated mutants for 2 h or 4 h. Cells were fixed and stained with DAPI. Representative confocal micrographs (a) and quantification (b) of GcAV formation. Scale bars: 10 μm. (c) Intracellular survival rate of GAS. The survival rates of GAS mutants at 2 h post infection were normalized to wild-type. (d) Autophagosome formation against GAS WT and Δnga in wortmannin-treated or non-treated (control) cells. (e) Western blot analysis of atg14 knockdown in HeLa cells. (f) Autophagosome formation against GAS WT and Δnga in control or atg14 knockdown cells. Data in (b, c, and d) were tested by two-tailed Student’s t-test: ***P < 0.001. NS, not significant.