Figure 2.

Y1H screen to identify regulators of ATG8B and ATG8E gene expression. (a) Promoter schematic of ATG8B and ATG8E genes. Red lines are fragments used for the Y1H screen. TSS indicates transcription start site. (b) Experimental design of Y1H assays. The library of TFs and ATG8 gene promoter fragments were introduced into yeast mating strains, diploids selected, and OD₆₀₀ and β-galactosidase reporter activity measured. (c) Venn diagram of the number of TFs identified for ATG8B and ATG8E promoters. (d) Comparisons between ATG8-Y1H data and DAP-seq data. DAP-seq TF binding profiles for 336 TFs in the TF library were used to assess the overlap between ATG8 promoter binding and Y1H targets identified in our screen. Overlap significance is shown as – log10(p-value) as calculated in GeneOverlap by Fisher’s exact test.