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. 2019 Apr 6;16(1):123–139. doi: 10.1080/15548627.2019.1598753

Figure 4.

Figure 4.

Enrichment of TF families and validation of Y1H candidates in Arabidopsis protoplasts. (a) Enrichment for TF families that bind to the ATG8B promoter. (b) Enrichment for TF families that bind to the ATG8E promoter. Families shown are statistically significant (p < 0.05), calculated using Fisher’s exact test. (c) Validation of Y1H candidates by transiently co-expressing each TF with an ATG8B promoter-luciferase fusion. (d) Validation of Y1H candidates by transiently co-expressing each TF with an ATG8E promoter-luciferase fusion. Candidates were selected based on TF family enrichment. The definitions and gene IDs are provided in Excel S1 for all the TFs shown in the figure. Dual-luciferase (REN and LUC) activities were monitored for each reaction. Data were normalized to a vector control for each promoter and shown as the average value ± SD of 3 biological replicates. Differences are significant at p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***) by Student’s t test.