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MIR5680 prevented VEC autophagy and apoptosis. VECs were treated with HG for 48 h after transfection with MIR5680 mimics or inhibitor overnight. (a) Western blot analysis of LC3B-II level. (b,c) Immunofluorescence analysis of the distribution of LC3B in VECs, and the proportion of cells containing > 5 LC3B puncta was analyzed. Scale bar: 20 μm. (d) TUNEL staining analysis of cell apoptosis. Scale bar: 50 μm. (*, p < 0.05; **, p < 0.01; n = 3.).
