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. 2020 Jan 27;15(1):e0226219. doi: 10.1371/journal.pone.0226219

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© 2020 Nagaya et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A. A flowchart for all samples’ analysis. The number of CTC detection and mRNA positivity are indicated in the box. Out of 203 CTC samples in total, 127 of them were positive for CTCs. B. 127 CTC-positive samples were classified according to the number of treatment when each sample was taken. Treatment lines were divided into two groups: pre 1^st-line/1^st-line or 2^nd-line/more. The proportions of positive mRNA expressions were compared between these two groups by the Fisher’s exact test. PSMA, AR, AR-V7, and EGFR were significantly more expressed in 2^nd-line/more group (P<0.05).