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Rapamycin was added to Pgal-eL43/L43 growing in galactose medium (no shift to glucose) and 4 hours later an extract was fractionated on a sucrose gradient centrifugation and aliquots of fractions were analyzed on a western blot developed with antisera specific to uL18/L5 and uL4/L4. All fractions from the top through the polysome region of the sucrose gradient are shown.
