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. 2020 Jan 27;15(1):e0222479. doi: 10.1371/journal.pone.0222479

Fig 7. Accumulation of extra-ribosomal uL18 does not require formation of the complete uL18-uL5-5SrRNA-Rrs1-Rpf2 complex.

Fig 7

Pgal-Rrs1 and -Rpf2 were grown in galactose medium and shifted to glucose medium for 16 hours. Whole-cell extracts were analyzed by sucrose gradient centrifugation and aliquots of two or three fractions from the top of the gradient and two fractions from the 60S-80S peaks were analyzed by western blots developed with antiserum specific to uL18/L5. Consecutive fractions from the top and the 60S-80S region of the sucrose gradient were shown.