Table 2.

Effects of vitamin C (VC) and cisplatin (Cis) alone and in combination on cell cycle of AGS cells

Sample	Controls	Cell cycle (% cells)					Cisplatin (µg/ml)	Cell cycle (% cells)
		Sub G0	G0/G1	S	G2/M			Sub G0	G0/G1	S	G2/M
VC (50 µg/ml)	RPMI control	p < 0.001	p < 0.001	Ns	Ns		2	p < 0.001	p < 0.001	Ns	p < 0.001
	Solvent control	p < 0.001	p < 0.001	Ns	Ns		10	p < 0.05	Ns	Ns	p < 0.05
VC (50 µg/ml) + Cis (2 µg/ml)	RPMI control	p < 0.001	p < 0.001	p < 0.01	Ns		2	p < 0.001	p < 0.001	Ns	p < 0.001
	Solvent control	p < 0.001	p < 0.001	Ns	Ns		10	p < 0.01	Ns	Ns	p < 0.05
VC (50 µg/ml) + Cis (10 µg/ml)	RPMI control	p < 0.001	p < 0.001	p < 0.001	Ns		2	p < 0.001	p < 0.001	Ns	p < 0.001
	Solvent control	p < 0.001	p < 0.001	p < 0.001	Ns		10	p < 0.001	p < 0.05	Ns	p < 0.01

Ns, non-significant difference (p > 0.05);   decreasing and   increasing cell percentage in the cell cycle phases because of cell treatments by samples in comparison with controls and Cis