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. 2019 Dec 27;9:20119. doi: 10.1038/s41598-019-56556-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SH-SY5Y cells were infected with ZIKV (MOI 10). After 24, 48, 72 and 96 hours of infection cellular viability was assessed by 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and analyzed by spectrophotometry. (a) SH-SY5Y cells were pre-treated (or not) with DHA (12,5 μM) before ZIKV (MOI 10) infection. After 96 hours, cellular viability was assessed by MTT assay and analyzed by spectrophotometry. (b) All MTT graphs were made using GraphPad Prism version 6.00. Additionally, SH-SY5Y cells were treated (or not) with DHA (12,5 μM) before ZIKV infection (MOI 10) and after 96 hours apoptotic and lytic cellular death was evaluated by staining cells with Annexin V and Propidium Iodide, analyzed thereafter by flow cytometry. (c) The dot plot is representative of three independent experiments and was made using FlowJo Version 9. A quantitative graph of Annexin-V/PI assay using data from three independent experiments was also performed. (d) All experiments here were performed in quintuplicate, each being repeated at least three times independently. P values are represented by asterisks: p ≤ 0.01 (**), p ≤ 0.001 (***) and p ≤ 0.0001 (****).