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. 2019 Dec 27;9:20119. doi: 10.1038/s41598-019-56556-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SH-SY5Y cells were pre-treated (or not) with DHA (12,5 μM) before ZIKV infection (MOI 10) and after 24, 48, 72 and 96 hours, inflammatory cytokines and chemokines released in the supernatant as MCP-1 (a), IL-6 (b), and TGF-β (c), was assessed by ELISA. Lipid inflammatory mediator Leukotriene B₄ was also assessed in the supernatant only at 96 hours of infection. (e) All the experiments above mentioned were analyzed by spectrophotometry and graphs were made using GraphPad Prism Version 6.00. Here, asterisks represent statistical significance with p ≤ 0.05 (*) and p ≤ 0.01 (**). All experiments here were performed in quintuplicate, each being repeated at least three times independently.