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. 2019 Dec 27;9:20119. doi: 10.1038/s41598-019-56556-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SH-SY5Y cells were pre-treated (or not) with DHA (12,5 µM) and after 24, 48 and 96 hours of infection, intracellular viral load was assessed by RNA extraction and analyzed by RT-qPCR. (a) This experiment was performed in triplicate. Additionally, SH-SY5Y cells pre-treated (or not) with DHA (12,5 µM) and after 96 hours of ZIKV infection, viral titers quantification was assessed by TCID-50 (50% Tissue culture Infective Dose), followed by further mathematical conversion to PFU. (b) This experiment was performed in sextuplicate. Asterisks represent statistical significance with p ≤ 0.01 (**) and p ≤ 0.0001 (****).