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. 2019 Dec 27;9:19868. doi: 10.1038/s41598-019-56375-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic setup of the Flu-SEC measurements. In this system, the continuous eluent flow is provided by an HPLC pump, and the fluorescently labelled sample is loaded in the system via an injector. The sample passing through the column is analysed with an on-line fluorescence detector directly connected to the column outlet and controlled by the software of the liquid chromatography system.