Figure 4.

Dependence of the peak height of the EV fluorescence peak from the number concentration of REVs using Alexa647-WGA (a) and PE-CD235a (b) for labelling at 0.2 μg/mL concentrations. Concentration series were measured between 3.54 · 10⁸ mL⁻¹ and 1.77 · 10¹¹ mL⁻¹. Individual replicates (N = 3) are shown for each concentration with symbols, and the best fitting linear functions are shown with solid lines on a log-log plot.