Figure 3.

ATG101 directly interacts with the PtdIns3K complex through its C terminus. GST proteins (4.5%) used in the binding assay and 20% of final ATG101-PtdIns3K complex binding product in vitro were subjected to the analysis as described in Materials and Methods. *, non-specific band (presumably IgG heavy-chain). (B) Quantification data for the interaction between ATG101 and PtdIns3K complex in presence or absence of HA-ATG14. Fold changes are represented by an assay background as a reference (mean ± S.D.; n = 3; **, P < 0.01; *, P < 0.05).