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. 2018 Aug 16;14(12):2104–2116. doi: 10.1080/15548627.2018.1504716

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Figure 4. — The WF finger motif of ATG101 is not responsible for binding to PtdIns3K complex. To determine the function of WF motif of ATG101, full-length wild-type Flag-ATG101 (Flag-ATG101-WPF) or full-length Flag-ATG101 mutants (Flag-ATG101 APA, Flag-ATG101 SPS) were co-transfected with PtdIns3K complex components (HA-ATG14 [A], HA-UVRAG [B], HA-PIK3C3 [C], and myc-BECN1 [D]) into HEK293 cells. For western blotting, cells were harvested and immunoblotted with the indicated antibodies. GAPDH was used as a loading control.