Figure 1.

Expression and localization of MAPT in developing neurons. (a) Distribution of MAPT in developing neurons determined by immunofluorescence. Rat hippocampal neurons cultured on coverglasses in 24-well plates were fixed and double stained for MAP2 (green) and MAPT (red). MAPT is distributed abundantly in the somata and processes of neurons at DIV3. (b) At DIV10, MAPT immunoreactivity disappears from the somatodendritic compartment and MAPT is sorted mainly to the axons. Scale bar: 20 µm. (c) Cartoon illustrating the transition in MAP2 and MAPT distribution from immature to mature neurons.