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. 2019 Dec 27;45(2):578–588. doi: 10.3892/ijmm.2019.4444

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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BCAR4 regulates Wnt/β-catenin signaling through miR-370-3p. (A) Putative binding sites for miR-370-3p on BCAR4 sequence and 3′UTR of Wnt7a mRNA. (B) Silencing of BCAR4 increased miR-370-3p levels in T24 and 5637 cells. (C) Silencing of BCAR4 decreased Wnt7a mRNA expression in T24 and 5637 cells. (D) Western blot analysis of Wnt7a protein expression in T24 and 5637 cells transfected with BCAR4 siRNA. (E) Quantitative analysis of Wnt7a protein expression in (D) showed that BCAR4 silencing reduced Wnt7a protein expression. (F) Transfection of miR-370-3p inhibitor decreased miR-370-3p in T24 and 5637 cells. (G) Silencing of BCAR4 decreased Wnt7a and increased p-β-catenin protein expression in T24 cells, which was reversed after miR-370-3p inhibition. (H) Quantitative analysis of Wnt7a and p-β-catenin/Total β-catenin protein expression in (G). (I) Silencing of BCAR4 decreased Wnt7a and increased p-β-catenin protein expression in 5637 cells, which was reversed after miR-370-3p inhibition. (J) Quantitative analysis of Wnt7a and p-β-catenin/Total β-catenin protein expression in (I). ^*P<0.05; ^**P<0.01; ^***P<0.001.

BCAR4 regulates Wnt/β-catenin signaling through miR-370-3p. (A) Putative binding sites for miR-370-3p on BCAR4 sequence and 3′UTR of Wnt7a mRNA. (B) Silencing of BCAR4 increased miR-370-3p levels in T24 and 5637 cells. (C) Silencing of BCAR4 decreased Wnt7a mRNA expression in T24 and 5637 cells. (D) Western blot analysis of Wnt7a protein expression in T24 and 5637 cells transfected with BCAR4 siRNA. (E) Quantitative analysis of Wnt7a protein expression in (D) showed that BCAR4 silencing reduced Wnt7a protein expression. (F) Transfection of miR-370-3p inhibitor decreased miR-370-3p in T24 and 5637 cells. (G) Silencing of BCAR4 decreased Wnt7a and increased p-β-catenin protein expression in T24 cells, which was reversed after miR-370-3p inhibition. (H) Quantitative analysis of Wnt7a and p-β-catenin/Total β-catenin protein expression in (G). (I) Silencing of BCAR4 decreased Wnt7a and increased p-β-catenin protein expression in 5637 cells, which was reversed after miR-370-3p inhibition. (J) Quantitative analysis of Wnt7a and p-β-catenin/Total β-catenin protein expression in (I). ^*P<0.05; ^**P<0.01; ^***P<0.001.