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. 2019 Dec 27;45(2):543–555. doi: 10.3892/ijmm.2019.4443

Figure 5.

Figure 5

miR-146a can directly target MED1. (A) Sequence alignment of MED1 3′-UTR and miR-146a in multiple species with seed sequences highlighted in red. (B) The luciferase activity of the reporter containing the MED1 3′UTR-WT or MED1 3′UTR-MUT was determined in AML12 cells transfected with NC or miR-146a mimic (n=3). (C-E) The protein level of MED1 (C) and densitometry analysis (D) and relative mRNA level (E) of MED1 in AML12 cells transfected with NC or miR-146a mimic, or transfected with scramble control or miR-146a inhibitor (n=3). (F-H) The protein level of MED1 (F) densitometry analysis (G) and relative mRNA level (H, n=5) of MED1 in the liver of normal diet mice (ND) or high fat diet mice (HFD). (I-K) The protein level of MED1 (I) densitometry analysis (J) and relative mRNA level (K, n=3) of MED1 in AML12 cells treated with BSA or FFA (1 mM). Means ± SD are shown. *P<0.05; **P<0.01; ***P<0.001, vs. NC, ##P<0.01, vs. scramble.