Figure 6.

ASB inhibits the NF-κB signaling pathway in UV-induced HaCaT cells. The cells were preincubated with ASB (10, 30 and 100 µM) and irradiated by UV (90 mJ/cm²). (A) Nuclear and cytoplasmic proteins were extracted; p65 and IκBα proteins were measured by western blotting. Relative changes in protein intensity were quantified for (B) IκBα, (C) cytosolic p65 and (D) nuclear p65 by densitometric analysis, and are presented as bar diagrams (n=3 for each group). ^#P<0.05, ^##P<0.01 vs. NC group; ^*P<0.05, ^**P<0.01 vs. UV-alone group. ASB, andrographolide sodium bisulfite; UV, ultraviolet; IκBα, NF-κB inhibitor-α; NC, normal control.