Table III.
Ingenuity Pathway analysis of upstream regulator activity inferred from differential target gene expression.
| Upstream TF | MB468 Hx
|
MB468 Nx
|
DLD Hx
|
DLD Nx
|
LNCaP Hx
|
LNCaP Nx
|
Entrez gene name | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fold ch | Z | Fold ch | Z | Fold ch | Z | Fold ch | Z | Fold ch | Z | Fold ch | Z | ||
| ATF3 | −2.000 | Activating transcription factor 3 | |||||||||||
| ATF4 | −1.091 | 1.186 | 2.000 | Activating transcription factor 4 | |||||||||
| CDKN2A | 0.447 | 0.000 | Cyclin-dependent kinase inhibitor 2A | ||||||||||
| CTNNB1 | −0.218 | −0.811 | Catenin (cadherin-associated protein), beta 1, 88 kDa | ||||||||||
| EHF | −0.302 | 0.632 | ETS homologous factor | ||||||||||
| ERG | 2.000 | V-ets avian erythroblastosis virus E26 oncogene homolog | |||||||||||
| ETV5 | −1.000 | Ets variant 5 | |||||||||||
| FOXM1 | 3.240 | −1.165 | Forkhead box M1 | ||||||||||
| FOXO1 | −2.221 | −2.621 | 1.442 | 1,969 | −1.131 | Forkhead box O1 | |||||||
| FOXO3 | −2.003 | −2.219 | −1.472 | −1.997 | Forkhead box O3 | ||||||||
| FOXO4 | −1.218 | Forkhead box O4 | |||||||||||
| GATA4 | −0.351 | GATA binding protein 4 | |||||||||||
| GLI1 | 0.114 | −0.671 | −1.400 | −1.154 | GLI family zinc finger 1 | ||||||||
| HAND2 | −0.351 | Heart and neural crest derivatives expressed 2 | |||||||||||
| HIF1A | −1.516 | −2.556 | −1.406 | −1.455 | −0.391 | −0.218 | Hypoxia inducible factor 1, alpha subunit | ||||||
| HLX | 1.000 | 0.447 | 0.378 | 0.000 | H2.0-like homeobox | ||||||||
| HOXD10 | 1.000 | 1.633 | Homeobox D10 | ||||||||||
| IRF3 | −2.208 | −1.972 | Interferon regulatory factor 3 | ||||||||||
| JUN | −1.474 | −1.622 | −1,653 | −1.000 | Jun proto-oncogene | ||||||||
| KDM5B | 0.000 | 0.832 | −2.864 | −0.816 | 1.667 | Lysine (K)-specific demethylase 5B | |||||||
| MDM2 | −1.192 | −2.000 | −0.686 | MDM2 proto-oncogene, E3 ubiquitin protein ligase | |||||||||
| MITF | 1.000 | Microphthalmia-associated transcription factor | |||||||||||
| MYC | 0.152 | 0.555 | −1,568 | 0.849 | −1.631 | 1.067 | V-myc avian myelocytomatosis viral oncogene homolog | ||||||
| MYOCD | −0.612 | Myocardin | |||||||||||
| NANOG | 0.707 | Nanog homeobox | |||||||||||
| NEUROG1 | 1.000 | 1.508 | Neurogenin 1 | ||||||||||
| NFIX | 1.407 | 0.655 | Nuclear factor I/X (CCAAT-binding transcription factor) | ||||||||||
| RELA | −2.564 | −2.386 | −1.913 | −0.849 | V-rel avian reticuloendotheliosis viral oncogene homolog A | ||||||||
| SATB1 | −1.494 | −0.576 | 0.984 | −0.831 | −1.987 | −1.987 | SATB homeobox 1 | ||||||
| SMAD4 | −1.000 | −1.987 | SMAD family member 4 | ||||||||||
| SMARCA4 | −1.980 | SWI/SNF related, matrix associated, actin dependentregulator of chromatin | |||||||||||
| SNAI2 | 0.854 | Snail family zinc finger 2 | |||||||||||
| SP1 | −1.000 | Sp1 transcription factor | |||||||||||
| STAT1 | −2.200 | Signal transducer and activator of transcription 1, 91 kDa | |||||||||||
| STAT3 | −1.653 | −0.864 | 0.640 | Signal transducer and activator of transcription 3 | |||||||||
| TBX5 | −0.351 | T-box 5 | |||||||||||
| TP53 | −2.756 | −2.568 | −1.480 | −0.651 | 0.492 | Tumor protein p53 | |||||||
| TP63 | 0.650 | 1.349 | 0.508 | −0.654 | 0.060 | Tumor protein p63 | |||||||
| TP73 | −1.230 | −0.970 | Tumor protein p73 | ||||||||||
| YAP1 | −1.633 | −1.633 | Yes-associated protein 1 | ||||||||||
TF, transcription factor; fold ch, fold-change; Z, z-score; siMB, cells transfected with small interfering RNA targeting myoglobin; MB+ cells transfected with scrambled small interfering RNA; Hx, hypoxia; Nx, normoxia.