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. 2019 Dec 18;45(2):510–518. doi: 10.3892/ijmm.2019.4431

Figure 4.

Figure 4

Effects of the SIRT1 inhibitor EX527 on NCM460 cell apoptosis. NCM460 cells were transfected with miR-195 antagomir or a scrambled oligo-nucleotide as a control before exposure to LPS. A total of 24 h after transfection, the cells were incubated with the SIRT1 inhibitor EX527 for 24 h. (A) Cell viability was measured using a CCK-8 assay kit. The SIRT1 inhibitor EX527 decreased the viability of NCM460 cells. (B) Apoptosis of NCM460 cells was determined by flow cytometry. (C) Quantification of NCM460 cell apoptosis. (D) Bax and Bcl-2 protein levels were measured by western blot analysis. (E) Bax and (F) Bcl-2 protein levels in NCM460 cells were determined and quantified. The data are presented as the mean ± standard deviation from three independent experiments. *P<0.05, **P<0.01. LPS, lipopolysaccharide; NC, negative control; PI, propidium iodide; SIRT1, sirtuin 1; miR-195, microRNA-195.