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. 2019 Dec 18;45(2):510–518. doi: 10.3892/ijmm.2019.4431

Figure 5.

Figure 5

miR-195 promotes apoptosis of NCM460 cells via targeting SIRT1/eIF2a. NCM460 cells were transfected with miR-195 antagomir or a scrambled oligonucleotide as a control. A total of 24 h after transfection, the cells were incubated with LPS. To further investigate the underlying mechanisms of miR-195, the cells were incubated with the SIRT1 inhibitor EX527 after transfection. (A) The expression levels of markers of the SIRT1/eIF2a signalling pathway were measured by western blot analysis. (B) Quantification of markers in the SIRT1/eIF2a signalling pathway. (C) A schematic figure demonstrating the roles of miR-195, Sirt and ER stress response. The data are presented as the mean ± standard deviation from three independent experiments. *P<0.05, **P<0.01. LPS, lipopolysaccharide; NC, negative control; SIRT1, sirtuin 1; miR-195, microRNA-195; p-, phosphorylated; eIF2a, eukaryotic translation initiation factor 2A; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; GADD34, growth arrest and DNA damage-inducible protein; ER, endoplasmic reticulum.