Figure 1.

Single-cell RNA-Seq reveals seven cell types of dissected mouse NH. A, Schematic representation of the scRNA-Seq procedure. Neurohypophyseal tissues were dissected from five C57BL6 adult male mice and pooled. Two independent pools were separately subjected to single-cell dissociation, single-cell capturing, and library preparation using the 10x chromium platform. The two libraries were then indexed and combined for sequencing using NextSeq 500 High Output v2 kit (75 cycles). B, The two libraries were pooled and mapped on the tSNE plot, showing cell clusters of IL cells, T-cell like, VLMC, epithelial like cells, vascular endothelia macrophage/microglia, and pituicyte. Each dot represents one cell, and cells with the same color belong to one cell type. C, Dendrogram showing the distance matrix from the PCA space of the average cell among the seven cell types. The length of the path between each two cell types indicates the relativeness between them. D, A table summarizing the number of cells, average number of genes and UMIs found in each cell type.