Figure 7.

Neurohypophyseal VLMCs are associated with fenestrated vascular endothelia. Confocal Z-stack (maximum intensity projection) of dissected NH, which was subjected to wholemount FISH with an antisense RNA probe directed to the VLMC marker, Lum, followed by immunostaining with an antibody directed to Plvap protein, which is a marker of fenestrated endothelia (scale bars, 100 µm). The bottom panels (labeled I–III) display high-magnification single plane confocal images of the respective regions delineated in white boxes in the top right panel (scale bars, 20 µm).