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. 2020 Jan 16;9:e52056. doi: 10.7554/eLife.52056

Figure 8. Effects of Gab1-knockdown on GSK3β and transcription factors.

Figure 8.

(A) OPC cultures were transfected with lentiviral vector-encoding with NC or shRNA, and the phosphorylation and expression of GSK3β were examined. GAPDH was the internal control. pGSK3β-S9p and Gab1 were 175 ± 16% (p=0.0063 vs control) and 27 ± 4% (p=0.00022 vs control) in shRNA compared to NC, respectively. n = 3/group. t-test, df = t(5). (B) Expressions of transcription factors in OPCs transfected with NC or shRNAs. Lysates were immunoblotted with antibodies against Sox10, Olig2, Mrf, Sox6 and Gab1. GAPDH was the control. Percentage changes in shRNA1 group are 58 ± 9% (Sox10) (p=0.0083 vs control), 38 ± 4% (Olig2) (p=0.0006 vs control), 16 ± 6% (Mrf) (p=0.00031 vs control), 209 ± 12% (Sox6) (p=0.00074 vs control), and 27 ± 3% (Gab1) (p=0.00022 vs control). n = 3/group. t-test, df = t(5). Gray dots indicate individual data points. ***p<0.001.