Key resources table.
| Reagent type |
Reagent or resource |
Source | Identifier | Additional information |
|---|---|---|---|---|
| Antibody | Rat monoclonal anti-Sst | Millipore | Cat#MAB354; RRID: AB_2255365 | (1:100) |
| Antibody | Guinea pig polyclonal anti-PV | Synaptic Systems | Cat#195004; RRID:AB_2156476 | (1:500) |
| Antibody | Donkey polyclonal anti-rat Alexa Fluor 488 | Jackson ImmunoResearch | Cat#712-545-150 | (1:500) |
| Antibody | Donkey polyclonal anti-guinea pig Alexa Fluor 488 | Jackson ImmunoResearch | Cat#706-545-148 | (1:500) |
| Antibody | Streptavidin Conjugated Alexa Fluor 488 | ThermoFisher Scientific | Cat#S11223 | (1:500) |
| Commercial assay, kit | iFX-enhancer | Invitrogen | Cat#I36933 | |
| Chemical compound, drug | Picric acid | Electron Microscopy Sciences | Cat#I9556 | |
| Chemical compound, drug | Glutaraldehyde | Sigma | Cat#G-7651 | |
| Chemical compound, drug | Sucrose | Sigma-Aldrich | Cat#S7903 | |
| Chemical compound, drug | Glucose | Sigma-Aldrich | Cat#G8270 | |
| Chemical compound, drug | Sodium chloride | Sigma-Aldrich | Cat#S9888 | |
| Chemical compound, drug | Potassium chloride | Sigma-Aldrich | Cat#P9333 | |
| Chemical compound, drug | Sodium phosphate monobasic dihydrate | Sigma-Aldrich | Cat#71505 | |
| Chemical compound, drug | Sodium bicarbonate | Sigma-Aldrich | Cat#S6297 | |
| Chemical compound, drug | Calcium chloride | Sigma-Aldrich | Cat#21115 | |
| Chemical compound, drug | Magnesium chloride | Sigma-Aldrich | Cat#68475 | |
| Chemical compound, drug | Cesium methanesulfonate | Acros Organics | CAS: 2550-61-0 | |
| Chemical compound, drug | HEPES | Sigma-Aldrich | Cat#H3375 | |
| Chemical compound, drug | EGTA | Sigma-Aldrich | Cat#E3889 | |
| Chemical compound, drug | Sodium phosphocreatine | Sigma-Aldrich | Cat#P7936 | |
| Chemical compound, drug | QX-314 (Lidocaine N-ethyl bromide) | Sigma-Aldrich | Cat#L5783 | |
| Chemical compound, drug | Magnesium-ATP | Sigma-Aldrich | Cat#A9187 | |
| Chemical compound, drug | Sodium-GTP | Sigma-Aldrich | Cat#G8877 | |
| Chemical compound, drug | Potassium gluconate | Sigma-Aldrich | Cat#G4500 | |
| Chemical compound, drug | DNQX | Tocris | Cat#0189; CAS: 2379-57-9 | |
| Chemical compound, drug | D-APV | Tocris | Cat#0106; CAS: 79055-68-8 |
|
| Chemical compound, drug | Gabazine (SR 95531 hydrobromide) | Tocris | Cat#1262; CAS: 104104-50-9 | |
| Chemical compound, drug | Clozapine-N-oxide | Enzo Life Sciences | Cat#BML-NS105 | |
| Chemical compound, drug | Phosphate Buffered Saline (PBS) Tablets, 100 mL | VWR | Cat#E404-200TABS | |
| Chemical compound, drug | Triton X-100 | VWR | Cat#0694–1L | |
| Chemical compound, drug | Normal Donkey Serum | Jackson Immuno-research Labs Inc | Cat#017-000 -121 |
|
| Chemical compound, drug | Bovine Serum Albumin | Sigma-Aldrich | Cat#A9647-100G | |
| Commercial assay, kit | Prolong Gold antifade reagent | Invitrogen | Cat#P36934 | |
| Other | Nail Polish | Electron Microscope Sciences | Cat#72180 | |
| Chemical compound, drug | Biocytin | ThermoFisher | Cat#28022 | 0.2–0.5% |
| Other | Raw and analyzed data | This paper | Available on github and by request | https://github.com/emguthman/Manuscript-Codes (copy archived at https://github.com/elifesciences-publications/Manuscript-Codes) |
| Strain, strain background (Mus musculus) | Mouse: C57BL/6J | Jackson Lab | RRID: IMSR_JAX:000664 | All sexes used |
| Strain, strain background (Mus musculus) | Mouse: Ssttm2.1(cre)Zjh/J | Jackson Lab | RRID: IMSR_JAX:013044 | All sexes used |
| Strain, strain background (Mus musculus) | Mouse: B6;129P2-Pvalbtm1(cre)Arbr/J | Jackson Lab | RRID: IMSR_JAX:008069 | All sexes used |
| Strain, strain background (Mus musculus) | Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Jackson Lab | RRID: IMSR_JAX:007905 | All sexes used |
| Strain, strain background (Mus musculus) | Mouse: B6N.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J | Jackson Lab | RRID: IMSR_JAX:026219 | All sexes used |
| Software, algorithm | pClamp 10.6 | Molecular Devices | RRID: SCR_011323 | |
| Software, algorithm | MATLAB_R2018a | Mathworks | RRID: SCR_001622 | |
| Software, algorithm | R | http://www.r-project.org/ | RRID: SCR_001905 | |
| Software, algorithm | Illustrator | Adobe | RRID: SCR_010279 | |
| Software, algorithm | Photoshop | Adobe | RRID: SCR_014199 | |
| Software, algorithm | SlideBook 6.0 | Intelligent Imaging Innovations (3i) | RRID: SCR_014300 | |
| Software, algorithm | Fiji | Fiji | RRID: SCR_002285 | |
| Software, algorithm | Grid/Collection Stitching | Fiji | RRID: SCR_016568 | |
| Software, algorithm | Neurolucida | MBF Biosciences | RRID: SCR_001775 |