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. 2020 Jan 8;9:e53003. doi: 10.7554/eLife.53003

Figure 2. Partial knockdown of MAP4K4 expression promotes oncogenic transformation and tumor formation.

(A) Schematic of experimental design to reveal binding proteins that when depleted can substitute for ST in transformation (B) Representative image of AI growth induced by ST or MAP4K4 partial knockdown (Grid shows 5 mm). (C) Quantification of AI growth following expression of MAP4K4 shRNA-82 (shMAP4K4), SV40 ST or corresponding controls (GFP or shLuc). Graph depicting tumor volume as a function of time (D) or an endpoint at day 28 (E) for subcutaneous xenografts expressing shLuc control or shMAP4K4-82 in HEK TER cells (Student’s t-test, **p<0.001, ***p<0.0001, ****p<0.00001).

Figure 2—source data 1. Quantification of soft-agar colony and tumor volume with MAP4K4 knockdown.

Figure 2.

Figure 2—figure supplement 1. Changes in AI growth with MAP4K4 knockdown.

Figure 2—figure supplement 1.

(A) AI colony count of HEK TER cells expressing the corresponding shRNAs or SV40 ST. Student’s t-test was performed for each shRNA compared to shLuc control. (B) AI colony count of HEK TER cells expressing eight different MAP4K4 shRNAs and MAP4K4 immunoblot showing the corresponding degree of MAP4K4 knockdown. The Student’s t-test was performed against shLuc control. (C) mRNA levels of MAP4K4 after knockdown using shMAP4K4-82 as measured by RNAseq (Transcript Per Million). (student’s t-test: **p<0.001, ***p<0.0001, ****p<0.00001).