Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mouse) | Atg7 | Ensembl:ENSMUSG00000030314 | ||
| Gene (Mouse) | Atg5 | Ensembl:ENSMUSG00000038160 | ||
| Strain, strain background (M. musculus, Male and Female) | C57/Bl6J | |||
| Strain, strain background (E. coli) | DH5α | ThermoFisher | Cat # 18265017 | |
| Genetic reagent (Mouse) | B6.Cg-Tg(Drd1a-tdTomato)6Calak/J | Jackson Laboratories | RRID: IMSR_JAX:016204 | |
| Genetic reagent (Mouse) | Tg(Adora2a-cre)KG139Gsat | MMMRC | RRID:MMRRC_031168-UCD | |
| Genetic reagent (Mouse) | Tg(Drd1-cre)EY262Gsat | MMMRC | RRID:MMRRC_030989-UCD | |
| Genetic reagent (Mouse) | Atg7tm1.1Tchi/Atg7tm1.1Tchi | Gift of Masaaki Komatsu | RRID:MGI:3590136 | (Komatsu et al., 2005) |
| Genetic reagent (Mouse) | 129S1/Sv-Hprttm1(CAG-cre)Mnn/J | (Tang et al., 2002) | RRID:IMSR_JAX:004302 | |
| Cell line (Mouse) | Atg5-/- transformed MEF | Gift of Ana Maria Cuervo; (Kuma et al., 2004) | RRID:CVCL_0J75 | |
| Cell line (Mouse) | Atg5+/+ transformed MEF | Gift of Ana Maria Cuervo; (Kuma et al., 2004) | ||
| Cell line (Mouse) | Atg7-/- primary MEF | This study | See Materials and methods | |
| Cell line (Mouse) | Atg7+/+ primary MEF | This study | See Materials and methods | |
| Antibody | Rabbit anti-Red fluorescent protein polyclonal | Rockland | Cat # 600-401-379 | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti-DARPP32 monoclonal | Cell Signaling Technology | Cat # 2306S | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-beta actin monoclonal | Novus Biologicals | Cat # NB600-501 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-Kir2.1 monoclonal | Antibodies Incorporated | Item # 73–210 RRID:AB_11000720 |
See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-Kir2.3 monoclonal | Antibodies Incorporated | Item # 75–069 RRID:AB_2130742 |
See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-Kv1.2 monoclonal | Antibodies Incorporated | Item # 75–008 RRID:AB_2296313 |
See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti-PSD95 polyclonal | Abcam | Cat # Ab18258 | See Table S3 in Supplementary file 1 |
| Antibody | Guinea pig anti p62 polyclonal | American Research Products | Cat # 03-GP62-C | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti p62 polyclonal | MBL | Cat # PM045 | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti LC3B polyclonal | Novus Biologicals | Cat # NB600-1384 | See Table S3 in Supplementary file 1 |
| Antibody | Chicken anti GFP polyclonal | Abcam | Cat # Ab13970 | See Table S3 in Supplementary file 1 |
| Antibody | Rat anti Lamp1 monoclonal | Iowa Hybridoma Bank | Cat # 1D4B | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti Kir2.1 polyclonal | Alomone Labs | Cat # APC-026 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti ubiquitin antibody monoclonal VU-1 | LifeSensors | Cat # VU101 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-acetyl-lysine monoclonal, clone 4G12 | Millipore | Cat # 05–515 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse Anti-Flag M2 monoclonal | Sigma | Cat # F1804 | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit anti-HA polyclonal | Abcam | Cat # ab9110 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-NeuN, monoclonal clone A60 | Millipore | Cat # MAB377 | See Table S3 in Supplementary file 1 |
| Antibody | Rabbit Anti-Atg7 (D12B11) monoclonal | Cell Signaling Technology | Cat # 8558 | See Table S3 in Supplementary file 1 |
| Antibody | Mouse anti-Tubulin (TU-01) | Invitrogen | Cat # 13–8000 | See Table S3 in Supplementary file 1 |
| Antibody | Pierce Protein A/G Magnetic Beads | Thermo | Cat # 88802 | See Table S3 in Supplementary file 1 |
| Antibody | Goat anti Guinea Pig IgG (H+L) Secondary antibody, Alexa 488 | Invitrogen | Cat # A-11073 | See Table S3 in Supplementary file 1 |
| Antibody | Goat anti Guinea Pig IgG (H+L) Secondary antibody, Alexa 647 | Invitrogen | Cat # A-24150 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa 488 | Invitrogen | Cat # A-21206 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa 594 | Invitrogen | Cat # A-21207 | See Table S3 in Supplementary file 1 |
| Antibody | Goat anti-Mouse IgG1 (H+L) Secondary Antibody, Alexa 488 | Invitrogen | Cat # A-21121 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Mouse IgG1 (H+L) Secondary Antibody, Alexa 594 | Invitrogen | Cat # A-21125 | See Table S3 in Supplementary file 1 |
| Antibody | Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa 488 | Invitrogen | Cat # A-11039 | See Table S3 in Supplementary file 1 |
| Antibody | Goat anti-Rat IgG1 (H+L) Secondary Antibody, Alexa 647 | Invitrogen | Cat # A-21248 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Rabbit IgG (H+L) Secondary IRDye 680LT | LI-COR | P/N 925–68023 | See Table S3 in Supplementary file 1 |
| Antibody | Streptavidin, Alexa 488 conjugate | Invitrogen | S11223 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Mouse IgG (H+L) conjugated to HRP | Jackson Immunoresearch | Code: 715-035-151 | See Table S3 in Supplementary file 1 |
| Antibody | Donkey anti-Rabbit IgG (H+L) conjugated to HRP | Jackson Immunoresearch | Code: 715-035-152 | See Table S3 in Supplementary file 1 |
| Recombinant DNA reagent | AAVDJ-CMV-DIO-Kir2.1-t2A-ZsGreen | Stanford Viral Vector Core (Rothwell et al., 2014) | AAV61 | |
| Recombinant DNA reagent | AAV2-EF1a-DIO-mCherry (3.2 × 1012 viral genomes/mL) | UNC Viral Vector Core | ||
| Recombinant DNA reagent | AAVDJ-hSyn-FLEX-mmAtg7-t2A-ZsGreen (1.7 × 1013 genome copies/mL) | Vector BioLabs | This study | |
| Sequence-based reagent | RNAScope Multiplex Fluorescent Reagent | Advanced Cell Diagnostics | Cat # 320850 | |
| Sequence-based reagent | RNAScope probe: Mm-Kcnj2 (Kir2.1) | Advanced Cell Diagnostics | Cat # 476261 | |
| Sequence-based reagent | RNAScope probe: Mm-Kcnj4 (Kir2.3) | Advanced Cell Diagnostics | Cat # 525181-C3 | |
| Commercial assay or kit | BCA protein assay kit | ThermoFisher | Cat # 23225 | |
| Commercial assay or kit | Immobilon Western Chemiluminescent HRP Substrate | Millipore | Can # WBKLS0500 | |
| Commercial assay or kit | XhoI | New England Biolabs | Cat # R0146S | |
| Commercial assay or kit | BamHI-HF | New England Biolabs | Cat # R3136S | |
| Commercial assay or kit | HindIII-HF | New England Biolabs | Cat # R3104S | |
| Commercial assay or kit | DpnI | New England Biolabs | Cat # R0176S | |
| Commercial assay or kit | Herculase II fusion DNA polymerase | Agilent Technologies | Cat # 600675 | |
| Commercial assay or kit | dNTP mix | ThermoFisher | Cat # R0191 | |
| Commercial assay or kit | Halt Protease Inhibitor Cocktail (100X) | ThermoFisher | Cat # 78430 | |
| Chemical compound, drug | Bafilomycin A1 | Tocris Biosciences | Cat # 1334 | |
| Chemical compound, drug | Tetrodotoxin citrate | Tocris Biosciences | Cat # 1069/1 | |
| Chemical compound, drug | Picrotoxin | Tocris Biosciences | Cat # 1128/1G | |
| Chemical compound, drug | SNAP-Cell 505-Star | New England Biolabs | Cat # S9103S | |
| Chemical compound, drug | SNAP-Cell TMR-Star | New England Biolabs | Cat # S9105S | |
| Chemical compound, drug | Neurobiotin tracer | Vector Laboratories | Cat # SP-1120 | |
| Chemical compound, drug | TrypLE | ThermoFisher | Cat # 12604054 | |
| Chemical compound, drug | Trichostatin A | Tocris Biosciences | Cat # 1406 | |
| Chemical compound, drug | Nicotinamide | Tocris Biosciences | Cat # 4106 | |
| Chemical compound, drug | N-ethylmaleimide | Sigma | Cat # E3876 | |
| Chemical compound, drug | PR-619 | Sigma | Cat # SML0430 | |
| Software, algorithm | Igor | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | pClamp | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Image Studio Lite | LI-COR | RRID:SCR_014211 | |
| Software, algorithm | Benchling | RRID:SCR_013955 | ||
| Software, algorithm | GraphPad Prism 7 | RRID:SCR_002798 | ||
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 |