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. 2020 Jan 21;7:478. doi: 10.3389/fbioe.2019.00478

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Copyright © 2020 Masaeli and Marquette.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Overview of the experimental set-up composed of a 12-well plate, 3D printed silicone positioning systems, GelMa coated circular glass slides and printed patterns of NIH3T3 cells. (B) Overview of the theoretical printing patterns in printer software. (C) Printed drops (15 nL) of alginate hydrogel used to setup size and distance between droplets. (D) Printed drops (15 nL) of NIH3T3 immediately after bioprinting. (E) Close-up image of the bioprinted nozzle filled with NIH3T3 cells. Scale bars: 500 μm.