Table III.

Buffer Capacities and Compositions of Bile Components and Phospholipids in of Some Common In Vitro Biorelevant Media and USP SIF, TS

BDM property	Value
	FaSSGF a,b	FaSSIFc	FaSSIF-V2d	FaSSIF-V3e		Bicarbonatef	USP SIF, TSg
Buffer species	–	Phosphate	Maleate	Maleate	Phosphate	Bicarbonate	Phosphate
Buffer pKa	–	6.69c	6.00e	6.00e	6.69c	6.04	6.69
Buffer concentration (mM)	–	28.7	19.1	10.26	13.51	16.2	50
pH	1.6	6.5	6.5	6.7		6.5	6.8
Osmolarity (mOsmol/kg)	120.7	270	180	215		Not measured	113
Experimental buffer capacity (mM/ΔpH)	–	12	10	5.6		7	18.4
Bile salt(s) (mM)	0.08 (TC)	3 (TC)	3 (TC)	1.4 (TC), 1.4 (GC)		–	–
Phospho-lipid(s) (mM)	0.020 (PC)	0.75 (PC)	0.2 (PC)	0.035 (PC), 0.315 (LPC)		–	–
Sodium oleate (mM)	–	–	–	0.315		–	–
Cholesterol (mM)	–	–	–	0.2		–	–
Average surface tension (mN/m)	42.6	54.7	54.2	35.1		Not measured	Not available

TC, taurocholate; GC, glycocholate; PC, phosphatidylcholine (lecithin); LPC, lysophosphatidylcholine (lysolecithin)

^aFrom ref. (19)

^bMedium also contains 0.1 mg/mL pepsin

^cFrom ref. (14)

^dFrom ref. (16)

^eFrom ref. (13)

^fFrom ref. (59)

^gFrom ref. (15)