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. 2020 Jan 27;10:1256. doi: 10.1038/s41598-020-58296-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of AnxA8 amplifies canonical Wnt signaling. (A) Western blotting reveals an increase in active β-catenin and GSK-3β protein in AnxA8-GFP transfected cells compared to controls, while β-catenin is unchanged (B) Quantification of blots such as those in A are presented as mean and standard deviation. Significant changes are marked with asterisks. *p ≤ 0.05. (C) AnxA8 overexpression in ARPE19 cells yielded increased levels of AnxA8 and slightly higher levels of GSK-3β mRNA. Addition of TGF-β1 led to increased β-catenin expression levels in AnxA8-overexpressing cells. Values are mean and standard deviation from 4 independent experiments determined by PCR. Asterisks indicate statistical significance. **p ≤ 0.01. (D) Transcripts of canonical Wnt target genes Axin1, c-myc and Lef1 were not significantly altered upon overexpression of AnxA8. Illustrated are mean and standard deviation of 4 separate data sets.