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. 2020 Jan 21;9:1565. doi: 10.3389/fonc.2019.01565

Figure 4.

Figure 4

EGFR was a direct target of miR-27a. (A) miR-27a inhibits the wild type but not the mutant EGFR 3'UTR luciferase activity. (B) EGFR was induced in chronic UVB irradiated mice skins. (C,D) the expression of EGFR in cSCC cells transfected with miR-27a mimic or inhibitor were detected by qPCR and western blot. (E,F) miR-27a suppresses the phosphorylation of EGFR and regulates its downstream pathways. miR-27a overexpression or inhibition of miR-27a regulated the phosphorylation of EGFR and the phosphorylation of p65, IκB, and IKK. Each experiment was performed in triplicate. Each experiment was performed in triplicate and data are presented as mean ± s.d. One-Way ANOVA and Dunnett's Multiple comparison test were used to analyze the data (*P < 0.05, **P < 0.01, ***P < 0.001).