Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 27;10:1194. doi: 10.1038/s41598-019-57279-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Epitope mapping through mutagenesis scanning of recombinant EGF receptor produced in HEK293-6E human cells. The reactivities of His-tagged mutated variants of human (A) and mouse (B) extracellular region of EGF receptor (erEGF-R) were evaluated on microtiter plates coated with nimotuzumab, cetuximab, K4 mAb and K5 mAb. Bound proteins were detected with an anti-His tag mAb followed by an anti-mouse Fc antibody conjugated to horseradish peroxidase. Relative reactivity (%) of each mutated protein was calculated taking the reactivity of the human non-mutated antigen as the reference. Arrows indicate loss of recognition of certain mutated variants.