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. 2020 Jan 27;10:1194. doi: 10.1038/s41598-019-57279-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of EGF receptor phosphorylation by antibodies. MDA-MB-468 (A) and H125 cells (B) were deprived of serum overnight and then incubated 2 h in the absence or presence of several concentrations of mAbs. Cells were stimulated with human EGF (100 ng/ml) for 10 minutes. Non-stimulated cells were used as negative control. Cell lysates were analyzed by western blot with antibodies to the Tyr 1068-phosphorylated form of EGF receptor (pEGF-R). Total EGF-R was also detected with specific antibodies.