Table 1.

Binding affinity and kinetic parameters of recombinant antibodies.

	kon (L/mol s)	koff (1/s)	KD (mol/L)	Increase
nimotuzumab	1.9 ± 0.2 × 104	1.3 ± 0.1 × 10−3	6.74 ± 1.1 × 10−8	—
K4 mAb	2.8 ± 0.5 × 104	6.1 ± 0.9 × 10−4	2.24 ± 0.7 × 10−8	3×
K5 mAb	3.3 ± 0.3 × 104	6.1 ± 0.5 × 10−4	1.86 ± 0.28 × 10−8	3.6×

Fab fragments from each antibody were obtained through papain digestion. These proteins were used for kinetic measurements by surface plasmon resonance on immobilized EGF receptor extracellular region recombinant protein. The values represent the mean ± SD calculated from four independent BIAcore experiments.