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. 2020 Jan 27;11(1):67. doi: 10.1038/s41419-020-2258-x

Fig. 3. Exogenous TCEA3 overexpression in RMS cell lines.

Fig. 3

a RH30, RH28, RD, and RD2 cell lines were transfected with a TCEA3-expressiion plasmid (pTCEA3) or the empty vector (EV). TCEA3 ectopic expression was confirmed by assaying TCEA3 mRNA expression by qRT-PCR. b Western blot with antibodies against TCEA3 was performed on the cells described in (a). to confirm the protein expression of TCEA3. GAPDH was used as the loading control. c TCEA3 immunofluorescence with antibodies against TCEA3 and DAPI used to stain nuclei on cells described in (a). Scale bar is 100 µm. Error bars are S.E.M. Student's t test; ***p < 0.001 with respect to EV, n ≥ 3 biological replicates.