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. 2020 Jan 27;11(1):67. doi: 10.1038/s41419-020-2258-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a RMS cells stably expressing TCEA3 or the vector control (EV) were seeded at the same number of cells and harvested for cell counts every 2 days. b Proliferation of RMS cells expressing TCEA3 was assayed by an EdU cell proliferation assay. Blue (DAPI) represents nuclei, and red represents EdU labeled nuclei, which labels cells undergoing DNA synthesis (S phase) during the time window of EdU treatment. Scale bar is 100 µm. EdU positive nuclei were counted in at least five random fields in microscopic images and percentage was calculated in comparison to total nuclei in the same images. Error bars are S.E.M. Student's t test; **p < 0.01, ***p < 0.001 with respect to EV, n ≥ 3 biological replicates except proliferation assay (n = 2).