Fig. 8. Overexpression of TCEA3 modulates RMS cell line sensitization to anticancer drugs.

a RH30, RH28, RD, and RD2 cell lines expressing TCEA3 (pTCEA3) or empty vector (EV) were seeded at 30 × 10⁴ per well in 6-well plates. After recovery for 24 h, cells were treated with 10 nM actinomycin D for 48 h. Viable cells were counted by trypan blue assay. b. TCEA3 overexpression sensitizes RMS cell lines to vincristine. Cells as in (a). were treated with 10 nM vincristine for 3 days, and then viable cells were counted by trypan blue assay. c TCEA3 overexpression sensitizes RMS cell linea to etoposide. Cells as in A. were treated with 50 μM etoposide for 24 h and viable cells were counted by trypan blue assay. d TCEA3 expression sensitizes RMS cell lines to anticancer drug combinations. Cells as in (a) were treated with combination of 10 nM actinomycin D with 50 μM etoposide or 10 nM vincristine with 50 μM etoposide for 24 h, and viable cells were counted using trypan blue. DMSO was used as a negative control. e RMS cell lines as in (a) were treated with TRAIL at 0.5 µg/ml. After 12 h, cells were trypsinized for trypan blue assay. Data are plotted as means with S.E.M. and analyzed with one-way ANOVA followed by Tukey’s HSD post-hoc test. Means not sharing same letter are statistically significant. n ≥ 3 biological replicates.