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. 2020 Jan 27;10:1245. doi: 10.1038/s41598-020-58005-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fenugreek increases overall intestinal microbial diversity. Fecal microbiome populations from CD, CD/FG, HFD, and HFD/FG mice were analyzed using 16S rRNA sequencing, and box plots were generated to depict differences in Shannon α-diversity. Data show that mice supplemented with 2% fenugreek in their feed exhibited a statistically significant (**p < 0.01) increase in α-diversity compared to mice given CD or HFD alone.