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. 2020 Jan 27;11(1):65. doi: 10.1038/s41419-020-2254-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The expression levels of TWIST1 in LoVo, HT-29, and HCT116 cells were detected by western blot following treatment with 3 ng/ml TGF-β or 2 μm LY 364947 for 48 h, *P < 0.05 and ***P < 0.001, versus control. b TWIST1 expression in LoVo, HT-29, and HCT116 cells transfected with NC-siRNA or TUG1 siRNA was analyzed by western blot, *P < 0.05, **P < 0.01, and ***P < 0.001, versus NC-siRNA. c, d Wound-healing assay (scale bar, 50 μm) and transwell invasion analysis (scale bar, 100 μm) of CRC cells transfected with TUG1 siRNA were transfected with NC-siRNA or TWIST1-siRNA.