Table 1.
Gene name | Accession number | Forward primer sequence | Reverse primer sequence | Probe |
---|---|---|---|---|
BCL-GS | NM_030766 | CAGGAGATCAGTTGGAAAGAAAG | CTGGTGTCAACCAAGGGAAT | 8 |
BCL-GL | NM_138722 | CCCCAGAGAATTCCTTGAGTC | TCATCATCATCTAGGGGGATTT | 85 |
BCL-GL | NM_138722 | GCTTTAAGGCTGCCCTTGTA | TCATCGGGTGGTTGTCAAT | 100 |
STAT1 | NM_007315 | GGATCAGCTGCAGAACTGGT | CTGTTCCAATTCCTCCAACTTT | 74 |
p65/RelA | NM_021975 | ACCGCTGCATCCACAGTT | GATGCGCTGACTGATAGCC | 47 |
BRM | NM_003070 | GATTCAGCCAGCACACTCCT | GGCGTGGACATCTACCTCTC | 53 |
BRG1 | NM_001128849 | TGGACCAGCACTCCCAAG | CTGGCTGGAACTGGACTAGAG | 21 |
LGR5 | NM_003667 | ACCAGACTATGCCTTTGGAAAC | TTCCCAGGGAGTGGATTCTAT | 78 |
MUC2 | NM_002457 | GCCAGCTCATCAAGGACAG | GCAGGCATCGTAGTAGTGCTG | 61 |
VIL1 | NM_007127 | GCAGCATTACCTGCTCTACGTT | GCTTGATAAGCTGATGCTGTAATTT | 71 |
ACTB | NM_001101 | ATTGGCAATGAGCGGTTC | CGTGGATGCCACAGGACT | 11 |
For BCL-G overexpression studies, relative mRNA levels of BCL-GL were measured using Universal ProbeLibrary RT-qPCR assay, probe 100.