Fig. 6. Change in contractility of the verapamil treated cultured cardiomyocytes.
a Representative real-time traces of change in sensor resistance ratio and cantilever displacement owing to the contraction and relaxation of different concentration of verapamil treated cardiomyocytes. b, c Bar plot depicting the cantilever displacement and relative contraction force generated by cardiomyocytes (**p < 0.01 measures by one-way ANOVA followed by Tukey’s honest significant difference test.). d Beat rate of cardiomyocytes at verapamil concentrations of 0.01 nM and 1 µM. e Beat rate of verapamil treated cardiomyocytes at different concentrations (0.01 nM–10 µM). f Change in resistance of the cardiomyocytes seeded cantilever at different verapamil concentrations (0.01 nM–10 µM). g Rise time and decay time of cardiomyocytes at verapamil concentrations of 0.01 nM and 1 µM. The rise time and decay time were analyzed and beat rate corrected with Fridericia’s formula (Rise time corrected (Rc) = R/interspike interval1/3) and (Decay time corrected (Dc) = D/interspike interval1/3), respectively. (**p < 0.01 measures by one-way ANOVA followed by Tukey’s honest significant difference test.). Error bars are mean ± s.d. (n = 5 biologically independent samples). NS and D represent displacement and nonsignificant, respectively.