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. 2020 Jan 27;11:535. doi: 10.1038/s41467-019-14019-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Displacement of the cantilever owing to the contraction and relaxation of the 1 µM verapamil treated cardiomyocytes at a different time before and after drug washed out. b, c Displacement of the cantilever after treating the cardiomyocytes with 1 µM verapamil. d Relative contraction force generated by 1 µM verapamil treated cardiomyocytes at different drug treatment time. e, f Rise time, and decay time of the 1 µM verapamil treated cardiomyocytes. The rise time and decay time were analyzed and beat rate corrected with Fridericia’s formula (Rise time corrected (Rc) = R/interspike interval^1/3) and (Decay time corrected (Dc) = D/interspike interval^1/3), respectively. (*p < 0.05, **p < 0.01 measures by one-way ANOVA followed by Tukey’s honest significant difference test. Error bars are mean ± s.d.). (n = 5 biologically independent samples).