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. 2020 Jan 27;11(1):69. doi: 10.1038/s41419-020-2266-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Immunoblot analysis of ATG12 protein in SETD2-deficient CAKI-1 cells and SETD2-competent ACHN cells, reveal the accumulation of both free ATG12 and of an additional ATG12-containing complex, distinct from the ATG5–ATG12 complex, in the SETD2-deficient RCC cells. b Immunofluorescence analysis shows an increase expression for ATG12 in CAKI-1 cells as compared with ACHN cells.