Fig. 4. VHL mutation in A498 and CAKI-2 RCC cells does not impact on the accumulation of free ATG12, and ATG12 forming complexes.

a Immunoblot analysis of ATG12 protein in CAKI-2 characterized by VHL gene mutation but a wild-type SETD2 gene, and A498 characterized by both VHL and SETD2 (loss of function) gene mutation revealed that the accumulation in free ATG12, and the ATG12-containing complex, distinct from the ATG5–ATG12 complex, is independent of the VHL gene status. b Immunoblot analysis, and its quantification in c, of ATG7 expression does not show striking differences between those two RCC cell lines. Bars represent the mean of three independent experiments, error bars represent SEM. Ns nonsignificant.