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. 2020 Jan 27;11(1):69. doi: 10.1038/s41419-020-2266-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–d Downregulation of SETD2 expression in the SETD2-competent ACHN cells was achieved by small-interfering RNA targeting SETD2. a Immunoblot analysis for SETD2 confirm the reduction in SETD2 expression in ACHN cells upon treatment with a siRNA directed against SETD2 for 48 h. b Immunoblot analysis of ATG12 protein reveals that the downregulation of SETD2 expression in the ACHN SETD2-competent cells promotes the increase in free ATG12 and ATG12-associated complexes. c Analysis of LC3B expression in upon downregulation of SETD2 expression. Bafilomycin A1 (40 nm) was used for 4 h treatment. d Quantification of LC3-II levels normalized to actin. Bars represent the analysis of three independent experiments, error bars represent SEM; ***p ≤ 0.001.