Figure 4.

Soluble ICOSL vIgD-Fc effectively attenuate T cell responses in mixed lymphocyte reaction (MLR). Monocyte derived dendritic cells from one donor were used to allogeneically stimulate purified T cells from another donor. Responses were measured by assessing (A) CD4 T cell proliferation, (B) CD8 T cell proliferation, and (C) IFNγ levels in culture supernatants. Proliferation results use CFSE dilution to show the percentage of divided cells vs. protein concentration. IFNγ levels are shown as pg ml⁻¹ vs. protein concentration, with IC₅₀ pM values calculated using GraphPad Prism and shown in parenthesis on graph label. ND, not determined. Results shown are representative of at least three separate experiments performed with each protein.