Fig. 2.

T-006 promotes α-syn degradationviathe proteasome. (a) Inducible PC12/A53T-α-syn cells were treated with or without 1 μg/ml Dox for 24 h, and then treated with 30 μΜ T-006, 30 μΜ CQ, 0.1 μΜ MG132 or 0.1% (v/v) DMSO for another 24 h. (b) Inducible PC12/A53T-α-syn cells were treated with 1 μg/ml Dox for 24 h, and then treated with 30 μΜ T-006 for different time periods. Representative Western blotting and data analysis of three independent experiments are shown. The proteasomal enzyme activities were measured using specific bioluminogenic enzyme substrates for chymotrypsin-like (c), trypsin-like (d), and caspase-like (e) activity. Data are represented as the mean ± SD. ^###P < 0.001 as compared to control group; *P < 0.05, **P < 0.01, ***P < 0.001, as compared to Dox group; ^$P <0.05, as Dox/CQ compared to Dox/CQ/T-006 group