Fig. 5.

T-006 promotes the degradation of Triton-soluble and -insoluble α-syn in the A53T-α-syn Tg mouse brain. Ten-month-old homozygous A53T human α-syn Tg mice (n = 5) were gavaged daily with T-006 (3 mg/kg) or olive oil (0.2 ml) for 4 weeks. (a) An experimental scheme. The cortex (b), midbrain (c) and striatum (d) fractions were homogenized and the protein level of Triton-soluble A53T-α-syn was analyzed by Western blotting. (e) Whole-brain levels of Triton-insoluble A53T-α-syn were analyzed by Western blotting. Representative Western blotting and data analysis of three independent experiments are shown. Data are represented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001